Composite

Part:BBa_I716218:Design

Designed by: Samantha Liang   Group: iGEM07_Berkeley_UC   (2007-06-26)


[rbs][ATG][lox][term][lox][BamHI]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 942
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 942
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 942
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 168
    Illegal BsaI.rc site found at 2029


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large) Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large) Product is pBca9145-I716218.


Source

Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large) Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large) Product is pBca9145-I716218.

References